Enzymatic Characterisation of Lignin Peroxidase from <i>Luffa aegyptiaca</i> Fruit Juice
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چکیده
منابع مشابه
Purification of a peroxidase from Solanum melongena fruit juice.
Solanum melongena fruit juice contains peroxidase activity of the order of 0.125 IU/mL. A method for the 11-fold purification of the enzyme was developed. The Km values of the peroxidase for the substrates guaiacol and hydrogen peroxide were 6.5 mM and 0.33 mM, respectively. The pH and temperature optima were 5.5 and 84 degrees C, respectively using guaiacol as the substrate. Sodium azide and p...
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Peroxidases have turned out to be potential biocatalyst for a variety of organic reactions. The research work reported in this communication was done with the objective of finding a convenient rich source of peroxidase which could be used as a biocatalyst for organic synthetic reactions. The studies made have shown that Luffa aegyptiaca (gourd) fruit juice contains peroxidase activity of the or...
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The purification of the lignin peroxidase from the juice of Musa paradisiaca stem using a simple procedure involving concentration by ultrafitration and anion exchange chromatography on DEAE cellulose column has been reported.The enzymatic properties of the purified enzyme have been found to be similar to the enzymes reported from the fungal sources.
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Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 micro...
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Phanerochaete chrysosporium BKM-1767 secretes multiple lignin peroxidase isoenzymes when grown under nitrogen-limited conditions. Here we report the purification of these heme-containing peroxidases, and their physical and catalytic charaterization. Ten hemeproteins, designed H1-H10, were separated by anion exchange HPLC. Six of them, H1, H2, H6, H7, H8, and H10, were lignin peroxidases, oxidiz...
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ژورنال
عنوان ژورنال: American Journal of Plant Sciences
سال: 2016
ISSN: 2158-2742,2158-2750
DOI: 10.4236/ajps.2016.73057